See more of the proteome
CinderBio offers two unique HTA-Proteases that generate orthogonal data sets
How Vesuvius and Krakatoa are similar and different
CinderBio’s HTA-Proteases Vesuvius and Krakatoa are both classified as ‘thermopsin’ proteases and derived from different species of acid and heat loving Archaea. Both enzymes show a cleavage preference for E, L, and F amino acids and result in significantly different peptide and protein identifications. Digestion of K562 cell extracts reveals only 11% of peptide and 63% of protein identifications are common to both enzymes. Vesuvius and Krakatoa are compatible with simultaneous co-digestion and sequential digestion of the same sample.
HTA-Proteases are shipped ready-to-use, store ambiently for >2 years, and reaction conditions (80° C, pH 3) obviate the use of chaotropes and alkylating agents. Both HTA-Proteases are particularly effective for membrane and nucleoproteins and have no observable autolysis.
Our enzymes are stored at ambient temperature, and we ship directly to our customers.
For a quote, please contact us at sales@cinderbio.com or simply order above using a credit card.