Krakatoa ~100 reactions
HTA-Protease© with E,L,F preferred cleavage sites. Optimal activity at pH 3.0, 80°C.
Reference #: HTA-KA-PC100 (CB23726)
Class: HTA-Protease
Concentration: 10U/uL
Volume (uL): 50uL
Buffer: Phosphate Citrate
Optimal pH: 3.0
pH Range: 1.8-4.2
Optimal Temp: 80°C
Temp. range: 40-100°C
Shelf Life: >2 years (ambient)
Krakatoa© is shipped ready-to-use, requires no aliquoting, and is stored ambiently. Krakatoa cuts at E, L, and F residues preferentially. No autolysis is observed when using Krakatoa on its own or when combined with Vesuvius HTA-Protease©. HTA-Protease digestions should be conducted at 70-90°C and pH of 2-4 for 5-90 minutes and do not require chaotropes, sample manipulation, or carbamidomethylation. Using brief one-step HTA-Protease reaction protocols significantly increase proteome and protein sequence coverage with datasets orthogonal to trypsin and have shown a bias for ribonucleoproteins, histones, mitochondrial, and membrane proteins.
DO NOT FREEZE. Store enzymes at ambient temperatures. Enzyme preparations are stored in aqueous buffered solutions at pH 3 with 20mM potassium phosphate dibasic and 40mM citric acid. Optimal shelf lives are observed in this buffer at ambient temperatures. Because of this storage condition, reasonable care should be taken to avoid microbial contamination of the enzyme aliquot.
Each order includes one 500U tube of Krakatoa and one 1mL tube of 10x PC Protease buffer.
To inquire about this product, request custom order quantities, or receive troubleshooting advice, please complete the inquiry form below or email info@cinderbio.com.