Vesuvius CB14057HC ~200 reactions
HTA-Protease© with E,L,F preferred cleavage sites. Optimal activity at pH 3.0, 80°C.
Reference #: CB14057
Class: HTA-Protease
Concentration: 30U/uL
Volume (uL): 100uL
Optimal pH: 3.0
pH Range: 1.5-4.0
Optimal Temp: 80°C
Temp. range: 38-100°C
Shelf Life: >2 years (ambient)
Vesuvius HTA-Protease© is shipped ready-to-use, requires no aliquoting, and is stored ambiently. Vesuvius cuts at E, L, and F residues preferentially. No autolysis is observed when using Vesuvius on its own or when combined with Krakatoa HTA-Protease©. HTA-Protease digestions should be conducted at 70-90°C and pH of 2-4 for 5-90 minutes and do not require chaotropes, sample manipulation, or carbamidomethylation. Using brief one-step HTA-Protease reaction protocols significantly increase proteome and protein sequence coverage with datasets orthogonal to trypsin and have shown a bias for ribonucleoproteins, histones, mitochondrial, and membrane proteins.d membrane proteins.
Each order includes one 3000U tube of Vesuvius-HC and one 1mL tube of 10x HTA-Protease buffer.
DO NOT FREEZE. Store enzymes at ambient temperatures. Enzyme preparations are stored in aqueous buffered solutions at pH 3 with 20mM potassium phosphate dibasic and 40mM citric acid. Optimal shelf lives are observed in this buffer at ambient temperatures. Because of this storage condition, reasonable care should be taken to avoid microbial contamination of the enzyme aliquot.
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