Vesuvius HTA-Protease© is shipped ready-to-use, requires no aliquoting, and is stored ambiently. Vesuvius cuts at E, L, and F residues preferentially. No autolysis is observed when using Vesuvius on its own or when combined with Krakatoa HTA-Protease©. HTA-Protease digestions should be conducted at 70-90°C and pH of 2-4 for 5-90 minutes and do not require chaotropes, sample manipulation, or carbamidomethylation. Using brief one-step HTA-Protease reaction protocols significantly increase proteome and protein sequence coverage with datasets orthogonal to trypsin and have shown a bias for ribonucleoproteins, histones, mitochondrial, and membrane proteins.d membrane proteins.
