Accelerating breakthroughs in Proteomics
CinderBio enables deeper discovery with new Archaeal enzymes, providing additional proteome coverage.
5min
Single-step sample preparation
100%
De novo antibody sequence with one enzyme in one run
>50%
Additional sequence coverage for known IDs
1 hour
From sample to novel biomarkers
CinderBio offers scientists a better way with a new class of heat and acid stable HyperThermoacidic Archaeal enzymes that reveal much more of the proteome while significantly shortening sample prep and digestion times. Derived from archaea that thrive in the acidic waters of volcanic hot springs, these enzymes are the key to unlocking new discoveries.
Overview
A new generation of proteases
For decades, Proteomics had relied on mesophilic enzymes and their multi-step, multi-day sample preparation before realizing results. These standard approaches are limited in their coverage, redundancy, and reach.
HTA-Protease Advantages
Antibodies
Histones
Nucleic acid-binding proteins
Mitochondrial and membrane proteins
Novel biomarkers
HTA-Proteases vs Standard Proteases
Detect more—faster and easier
Get faster and better results with the same LC-MS/MS process
Peer Reviewed Results
Research Partners
Funding Partners
Highly efficient hyperthermal acidic proteases combined with hybridfragmentation schemes provide five times more unique peptide reads than trypsin or chymotrypsin, greatly boosting confidence in de novo sequencing. This approach enables confident de novo antibody sequencing based on a single LC-MS run.
IN BRIEF
Laura Perez Paneda, Tereza Kadava, Tatiana M. Shamorkina, ..., Narlock-Brand, Steven M. Yannone, Joost Snijder, and Albert J.R. Heck
AUTHORS
JOURNAL
Deep coverage and extended sequence reads obtained with a single archaeal protease expedite de novo protein sequencing by mass spectrometry
Antibody Sequencing
March 11, 2026
High Throughput Proteomics
Recent Peer-Reviewed Papers
From volcanoes to the bench: Advantages of novel HyperThermoacidic Archaeal proteases for proteomics workflows
October 30, 2023
Novel HTA-proteases function at 70–90°C and pH 2–4, enabling a rapid, 5-minute, single-step preparation for bottom-up proteomics without chaotropes. Their unique cleavage expands sequence coverage for challenging proteins, offering significant advances for high-throughput and clinical proteomics.
Maxwell C. McCabe, Varun Gejji,, Adam Barnebey , Gary Siuzdak , ..., Saviola, Steven M. Yannone, Kirk C. Hansen
AUTHORS
IN BRIEF
JOURNAL
Slow tryptic digestion limits clinical proteomics. The novel "Krakatoa" protease solves this, digesting samples in 5-30 minutes. Combined with rapid LC-MS, it quantifies over 150 proteins and key peptides from raw biofluids in under an hour, enabling true real-time clinical analysis.
IN BRIEF
Steven M. Yannone, Vikas Tuteja, Olena Goleva, Donald Y. M. Leung, ..., Nathan G. Hendricks, Sarah Parker, Jennifer E. Van Eyk, and Simion Kreimer
AUTHORS
JOURNAL
Toward Real-Time Proteomics: Blood to Biomarker Quantitation in under One Hour
Biomarker Discovery
Difficult Proteins
March 20, 2025
High Throughput Proteomics
High Throughput Proteomics
Difficult Proteins
How To Use
Our Products
Join the proteases revolution and see more biology
CinderBio offers two unique enzymes that can be used together for maximum proteome coverage