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Enzymes for Proteomics & Industry


About Our Products

Volcanic enzymes for bottom-up proteomics

CinderBio's patented technologies allow us to produce new heat and acid stable HTA-enzymes for bottom-up proteomics and industrial applications that work faster and last longer than anything previously available. These unique enzymes are made from amazing microbes that thrive in hot volcanic waters around the globe.

These enzymes provide access to parts of the proteome unavailable with standard tryptic digest protocols. Sample preparation in as fast as 5 minutes[1] can be achieved allowing for efficiency in workflow turnaround times which make acute-care[2] clinical proteomics applications possible.

How is it different?

  • Both Krakatoa and Vesuvius are semi-specific proteases (E, L, or F) which digest at high temperatures in acidic conditions (e.g., 80C, pH 3)

  • Due to the heat and acid conditions, no chaotropes are required for digestion

  • Alkylation is also not required as acid conditions ensure cysteines remain protonated

  • For more detail read the paper and the latest protocol

Example applications:

  • Desired bias for ribonucleoproteins, histones, and mitochondrial membrane proteins

  • Denovo sequencing of single proteins (e.g., antibodies)

  • Fully automated sample prep system

  • High sequence coverage using a simple protocol (e.g., histones)

We're constantly discovering new enzymes to enhance our products. Stay tuned for updates!

[1] M.C. McCabe, V. Gejji, A. Barnebey, G. Suizdak, L.T. Hoang, T. Pham, K.Y. Larson, A.J. Saviola, S.M. Yannone, K.C. Hansen, From volcanoes to the bench: Advantages of novel hyperthermoacidic archaeal proteases for proteomics workflows, J Proteomics  (2023) 104992

[2] Acute-care clinical proteomics applications are defined as requiring less than one hour total turn-around time.



Welcome to CinderBio, where we’re at the cutting edge of proteomics allowing you to discover the world of unique proteins/protein sequence to advance precision medicine. We unlock access to parts of the proteome unobserved with parallel standard methodologies. Our enzymes allow for the speed required to enable near real-time analysis to the bedside
Let us show you how our unique coverage of proteins allow you to discover new biological insights and advance your science.

Our enzymes provide unique protein coverage

Protein coverage with trypsin alone vs. trypsin in combination with HTA-protease data.

Proteins with <20% coverage improvement are plotted in grey, proteins with >20% coverage improvement are colored by class: nucleic acid-binding (magenta), mitochondrial (yellow), chaperone (purple), nuclear (orange) and other (white). The dashed line indicates the 20% improvement boundary.

Reveal an additional 20% of the Proteome

Incremental coverage of the proteome.  Cumulative unique amino acid coverage from HTA digest.


CinderBio’s innovative technologies and business model have been recognized in national business plan competitions, competitive commercialization programs, and Federal research funding agencies:


CinderBio Products

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